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Departamento de Química Biológica

C Oil extraction well next to the stream in the 30s photograph by Pedro Penna, Parsons The TPH concentrations in the stream at the oil spill discharge zero meters were Hence, patterns of multiple CYP genes expression in rainbow trout gills and liver could be especially valuable for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems.

TPH concentration in water was 4. Informe, Buenos Aires, Argentina, 64 pp. We found induced CYP3A27 transcript levels only in liver of impacted wild trout, suggesting an induction at longer time than four days. The annealing temperatures and primer pairs used during the duplex PCR for each gene are shown in Table I.

Biomarker induction in tropical fish species on the northwest shelf of Australia by produced formation water. Liver and gills from four fish were aseptically dissected out and pooled into one sample of each organ, obtaining a total of three pooled samples per organ and per reach.

Effects on circulating hemocyte integrity and immune related functions were mostly transient or remained stable within 7 days; except for increased lysosomal labialization and decreased extracellular ROS production when mussels were exposed to the toxin combination.

Samples were diluted with ultra-pure water to adjust to a concentration of 2. Data was complemented with laboratory exposure to water accommodated fraction WAF from the oil spill. Excretion of aromatic hydrocarbons and their metabolites by freshwater and seawater Dolly Varden char. Mar del Plata, Argentina, Aprilpp. Nevertheless, diet and transplantation procedures may produce negative effects on the oxidative balance of gills and digestive gland and should be taken into account for bioremediation strategies.


Both compound classes caused paralysis and immune impairment. Bands were analyzed densitometrically using Image J software. However, there are few studies on the nature of PXR ligands in fish Ekins et al.

Lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure. In order to test if orally supplied Euglena sp. The spills are the relict of a wellbore, an attempt to have oil production during the early 20th century and then abandoned Mancini and Serna The cytochrome P superfamily: These clear and consistent changes reaffirm CYP1A transcript expression as a good semiquantitative acuztica for crude oil exposure.

The transcript levels of CYP2M1 declined 1. This well-preserved low-order stream is particularly suitable for oil exposure biomarker studies since the control and the impacted areas are consecutive reaches, thus, the studied individuals belong to the same population, and the oil spill is the only cause of water quality change. Letters denote significant differences among treatments within each time, 24 h lowercase letters and 96 h capital letters from Bonferroni test Table II.

Antioxidant catalase and detoxifying gluthatione S-transferase defenses are generally lower in gills and digestive gland of EG mussels than in SV ones. Nuclear receptor-mediated toxicity of xenobiotics. Standardization of the preparation and quantitation of water-accommodated fractions of petroleum for toxicity testing.

Cytochrome P CYP in fish. Editado por TW Parsons, Bariloche, pp. A Oil remains afloat on the surface of stream ecotoxicolgoia.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

In contrast, gill CYP1A transcripts returned to control levels before 96 h. Since oil exploration and extraction ecotoxiclogia are expected to be enhanced in this region in the future, further studies and monitoring programs are needed to protect the quality of valuable water ecosystems, fish production in downstream reservoirs and human health.

Functional characterization of a full length pregnane X receptor, expression in vivo, and identification of PXR alleles, in Zebrafish Danio rerio. The application of toxicity test on the specie Atriplex lampa in the risk assessment of oil spills in Patagonian soils. Julieta Soledad De Anna.


The upstream ecotoxicplogia is up of a 1.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

This explains the similar response in liver CYP1A transcript levels at 24 and 96 h during our experiments, without dose-dependent effects. Triplicate samples were collected at the upstream reach —, — and — mat the oil spill zero metersat the downstream reach 50, and m and further downstreamand m.

The spills converge into a unique source point of pollution, with liters of crude oil per day entering the stream in February Comparison of rainbow trout and mammalian cytochrome P enzymes: Gene transcript expression of several ecotoxicooogia P oxidases was studied by semi-quantitative PCR in both field and laboratory.

Fish ecotoxucologia killed and dissected as described above for field samples. Hemocytes’ viability is increased for E. The study of mRNA expression patterns of multiple CYP genes in rainbow trout gills and liver would be extended in order axuatica evaluate their use for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems.

Fish are represented by a single species, the exotic rainbow trout O. CYP3A27 levels were only induced in liver of wild trout 1. First, we explored the heavy crude oil effects on these possible biomarkers in wild juvenile rainbow trout that were caught in the La Mina stream, up and downstream of the oil spill. This impairs juvenile fish migrate from the downstream to the upstream reach, but it might not necessarily prevent migration from the upstream to the downstream reach.

The advance of these activities can endanger Patagonian aquatic ecosystems, which are very well preserved until now.

The phase I xenobiotic metabolizing enzymes ecotxicologia represented mainly by cytochrome P CYP multi-enzymatic complex, achatica catalyzes hydrolysis, oxidation and reduction reactions, by adding or acuatiica functional groups Danielson

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