Indian J Physiol Pharmacol. Oct;45(4) In vivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves. Singh B(1). Request PDF on ResearchGate | Hepatoprotective activity of Eclipta alba Hassk. against paracetamol induced hepatocellular damage in mice | The effect of. The hepatoprotective activity of different extracts of whole plant Eclipta alba was investigated against CCl4 induced hepatic damage inmale albino rat.
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KSaxena R. Biomed Pharmacol J ;1 1. Hassk has been widely used in India for the traditional treatment of liver disorders Chopra et al. Mehra and Handa, An Ayurvedic drug from Eclipta alba has been found to be quite beneficial for the treatment of jaundice in children Dixit and Achar, In vitro immuno-inactivation of surface antigen hepatoprtective hepatitis B virus HBsAg by Eclipta alba has been reported Thyagarajan et al.
This plant showed antihepatotoxic activity in assays using CCl4 galactosomine and phalloidin — induced cytotoxicity in cultured rat hepatocytes Wagner et al. Shrivastava performed taxonomic identification and the voucher specimen was deposited in the herbarium of our laboratory for future reference vide access no. The plants were washed thoroughly with tap water and air dried in shade at room temperature. They were then mechanically powdered and sieved.
Another gm of the powdered plant material was decocted in a ml of water. The extractive yield were found to be A preliminary phytochemical screening was carried out for the extracts employing the standard procedure to reveal the presene hepatoprotecive alkaloids, steroids, terpenes, flavonoid, saponins, tennins, glycosides, carbohydrates and proteins.
Twenty four albino rats of wistar albino rats weighing gm were obtained from laboratory of college. They were fed with pallets of Golden feed, New Delhi.
Water was supplied libitum. They were given a week time to get acclimatized with the laboratory conditions. Rats were divided in to six groups of 6 rats each as follows.
All the group were given fresh food daily at Animals were kept starved over night on 90 th day. On the next day the blood was collected by making an incession by jugular vein to collect the blood.
The liver tissue was dissected by blotting of the blood washed in saline. Serum was prepared from the collected blood and subjected to biochemical estimation of different parameters like aspartate aminotransferase ASTalanine aminotransferase ALTalkaline phosphatase ALPtotal lipid, triglceride, phosphatase and cholesterol.
Liver homogenates were also subjected to various biochemical estimation. After paraffin embedding block, were stained with Haemotoxylin and Eosin and examined under microscope. A few photomicrographs of representative types were also taken. One way analysis of variance ANOVA followed by Scheffes test was applied for determinig the statistical significance of difference in enzymes, protein and lipids levels between different group.
The level of significance was set at 0. Total biochemical parameters was recorded in the present study.
The possible hepatoprotective activity of Eclipta alba whole plant extract against CCl4| Abstract
Values were little less as compare to the Silymerin. Regarding ALP the value remained to be a bit higher as compared to the control group and Silymerin treatment. As regards the total proteins the mg dose of Eclipta alba brings the protein value at most equal to the normal rats. As regards the total lipid the value got increased in 50 mg dose but at higher dose, it got decreased which is still much higher then the total lipid values in the control rats.
The possible hepatoprotective activity of Eclipta alba whole plant extract against CCl4
Cholesterol value came to be nearer to the control group of animals. There was a considerable increase in the phospholipids of the plant extract treated group VIth serum as compared to the group 1 which recorded only LP liquid paraffin.
Elipta, it is quite clear that, total lipid, cholesterol and phospholipids were found to be increased against the control group Ist.
Rest parameters were found to be all most normal level when three different doges of the compound of the Eclipta alba were given to the CCl4 induced hepatotoxic rat. The level of total protein was found to be at normal level after the treatment. Similar akba have been observed by Pattanayak and Priyashree which have noticed such changes in the hepatotoxic animal when treated with the extract from Dendrophoe falcanta. Similarly, level of hepatoprotwctive, total lipid and cholesterol was found to be decresed after the treatment.
Vanukumar and Latha have also reported that when liver cell plasma membrene is damaged, a variety of enzymes normally located in the cytosol which are released in blood stream which causes hepatocellular changes.
Treatment of Axtivity increases the level of total proteincholesterol in the liver.
In vivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves.
But it was noiticed that the recovery beginning as soon as the plant extract was given. The phospholipids content in serum registered a significant like that of liver showed a deamination in CCl4 administered group. A histopathological study of the liver further suggest the hepatoprotective efficacy of Eclipta alba extracts fig.
Suresh Kumar and Mishra have also reported hepatoprotective effect of Pergularia demia ethanol extract. The observations of these dose, clearly indicat the involvement of heptoprotective active principles in Eclipta alba. The detail phytochemistry of the active hepatoprotective principles is still awaited. This work is licensed under a Creative Commons Attribution 4. February 12, Manuscript accepted on: April 04, Published online on: Copy the following to cite this article: Copy the following to cite this URL: