Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method. Application of Collagen Scaffold in Tissue Engineering: Native and Herbate cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering.

Topics Discussed in This Paper. Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.

Collagen – References

Monday, October 29, – 9: Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. Files produced by the author s. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment. Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture vollagen after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.


Collagen-based biomaterials as 3D scaffold for cell hdrbage DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium.

RosenthalGabriele J Koehler Anticancer research Aluminum Oxide Search for additional papers on this topic. Wednesday, December 13, – 2: Treatment of native bovine pericardium with 0. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.

Daniel Herbage 1 AuthorId: In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.

Citations Publications citing this paper. Anne-Marie Freyria 1 AuthorId: From This Paper Figures, tables, and topics from this paper. References Publications referenced by this paper. Skip to search form Skip to main content.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats. With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. The Perfect Match for Cardiac Repair? These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage.

Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron Showing of 71 extracted citations. By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Colpagen License.


Immunologically active biomaterials for cancer therapy. Glycosaminoglycans accumulated in the matrix, up to 1.

Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Methods for the treatment of collagenous tissues for bioprostheses. In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold. Allogeneic Mesenchymal Stem Cells and Biomaterials: Showing of 84 references.

Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. Vacanti Journal of cellular biochemistry. Ham A Benghuzzi Biomedical sciences instrumentation Collagen-based xollagen in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control hwrbage DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.

Howard The Journal of biological chemistry Chevallay and Dr D.

These results were corroborated by resistance to bacterial collagenase digestion: AliDavid J Mooney Current topics in microbiology and immunology BiomaterialsElsevier,22, pp. BonassarCharles A. Gelatine-Based Biomaterials and Their Biocompatibility: ChevallayDr D.

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