Introducción a la HPLC: aplicación y práctica. Front Cover. Oscar Alberto Quattrocchi. Artes Gráficas Farro, – pages. 6 oct. We are always happy to assist you. Document Description. Introduccion a La HPLC Quattrocchi. Document Share. Documents Related. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in mL .. Quattrocchi OA, De Andrizzi SA, Laba RF. Introducción a la HPLC Aplicación y. Práctica. Buenos Aires: Artes Gráficas.
|Published (Last):||19 August 2011|
|PDF File Size:||4.48 Mb|
|ePub File Size:||20.90 Mb|
|Price:||Free* [*Free Regsitration Required]|
A carbon paste electrode was modified with the ionic liquid 1-methyloctyl imidazolium hexafluorophosphate and it was applied for study the electrocatalytic oxidation and voltammetric determination of the drug sulfamethazine. Acta8 Six patient volunteers who were using nifedipine mg once daily as treatment for hypertension participated in this study.
No interference was observed.
The accuracy and precision did not exceed 4. Best regards, Dmitriy A.
Three hplx each were prepared and each replicate was applied as bands three times. Calcium-channel blockers, as nifedipine, are considered one of several preferred antihypertensive drugs for the initial management of hypertension in patients with a high risk of developing coronary artery disease, including those with diabetes mellitus. The developed modified electrode was characterized using cyclic voltammetry and scanning electron microscopy.
Some wavelengths were tried, and nm was selected as working wavelength.
Chile, Care44 Services on Demand Journal. Trials2 ,S.
Introduccion a La HPLC Quattrocchi – Documents
Karimi, Talanta Drug-free serum used for the validation of the method was obtained from healthy volunteers. The organic layer was transferred to another glass tube and the contents were evaporated to dryness at room temperature under a gentle stream of pure nitrogen.
For determination of nifedipine in blood, some methods using HPLC, voltammetry and spectrophotometry 39,40 have been reported. Actuators B The method was sensitive enough to detect a quantity as low as 0. Sat Nov 30, 8: Chromatographic chambers were previously saturated with solvent mixture for 20 min. The migration distance was 8 cm and the migration time 12 min. The analysis is fast, and the method allows a large number of samples to be measured simultaneously.
The results are presented in Tables 1 and 2. Care37 Selectivity The selectivity of the method was examined by preparing and analyzing checked by analyzing human serum samples spiked with nifedipine and the internal standard carbamazepine. The more common ways to evaluate the adherence of the patients with their medication are questionnaires about the daily administration of the drug, and pill counts, with the problem that the results can be true or false.
Working solutions were prepared at concentrations of 0. Each solution was applied as bands 3 times.
Introduccion a La HPLC Quattrocchi
Inter-assay quattrcochi and inter-assay accuracy were calculated after repeated analysis in three different analytical runs. Pharmaceutics, Follow us on Twitter: Is an excellent book for beginners, the only drawback is that is rather old. Therefore, the principal advantage of HPTLC is a fast analysis, with h;lc high selectivity, accuracy, sensitivity nanograms in UV absorbance mode and picograms in fluorescence mode and precision.
Nifedipine is used alone or in combination with other classes of antihypertensive agents in the management of hypertension.
Application of the method. Calibration curves were constructed using peak-area ratio for the nifedipine and the itnroduccion standard carbamazepine as a function of the concentration added.
No other method using HPTLC was found in the literature, for the quantification of nifedipine in human serum neither in human plasma. The linear range was between 0. The volume of serum used was 2 mL.